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首页> 外文OA文献 >Identification of Sequence Diversity in the Escherichia coli fliC Genes Encoding Flagellar Types H8 and H40 and Its Use in Typing of Shiga Toxin-Producing E. coli O8, O22, O111, O174, and O179 Strains▿
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Identification of Sequence Diversity in the Escherichia coli fliC Genes Encoding Flagellar Types H8 and H40 and Its Use in Typing of Shiga Toxin-Producing E. coli O8, O22, O111, O174, and O179 Strains▿

机译：鉴定鞭毛类型为H8和H40的大肠杆菌fliC基因的序列多样性及其在产志贺毒素的大肠杆菌O8，O22，O111，O174和O179菌株的分型中的应用▿

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Flagellar type H8 is associated with many strains of pathogenic Shiga toxin-producing Escherichia coli (STEC), such as O8, O22, O111, O174, and O179 strains. Serological typing of the H8 antigen is limited to motile strains only and suffers from cross-reactivity between flagellar H8 and H40 antigens. In order to develop a method useful for typing of motile and nonmotile STEC O111 and other strains, we have analyzed the flagellar antigen (fliC) genes in representative E. coli H8 and H40 types. Two genotypes of the fliC gene encoding H8 (the fliC-H8 gene) were identified. Genotype fliC-H8a was found to be conserved in STEC O111, O174, and O179 strains; and type fliC-H8b was associated with STEC O8 and O22 strains. Sequence variations were also found in the genetically closely related fliC-H40 gene, although the latter was not found to be associated with STEC strains. A PCR was developed for the specific identification of the fliC-H8 and the fliC-H40 genes in motile and nonmotile E. coli strains. Digestion of PCR products with HhaI resulted in restriction fragment length polymorphisms (RFLPs) which were associated with genotypes fliC-H8a and -H8b as well as with genotypes fliC-H40a and -H40b. The fliC-specific PCR/RFLP typing method was suitable for the rapid typing of motile and nonmotile STEC O8, O22, O111, O174, and O179 strains from different sources whose fliC-H8 genotypes were found to be highly conserved. The fliC genotyping method is advantageous over serotyping and is useful for epidemiological investigations and studies of the evolution of STEC clones.
机译：H8型鞭毛与许多致病性产志贺毒素的大肠杆菌（STEC）菌株相关，例如O8，O22，O111，O174和O179菌株。 H8抗原的血清学分型仅限于活动株，并遭受鞭毛H8和H40抗原之间的交叉反应。为了开发一种可用于对运动型和非运动型STEC O111和其他菌株进行分型的方法，我们分析了代表性大肠杆菌H8和H40类型的鞭毛抗原（fliC）基因。鉴定了编码H8的fliC基因的两种基因型（fliC-H8基因）。发现基因型fliC-H8a在STEC O111，O174和O179菌株中是保守的。 fliC-H8b型与STEC O8和O22菌株有关。在遗传上密切相关的fliC-H40基因中也发现了序列变异，尽管未发现后者与STEC菌株相关。开发了一种PCR，用于特异性鉴定活动和非活动大肠杆菌菌株中的fliC-H8和fliC-H40基因。用HhaI消化PCR产物会导致限制性片段长度多态性（RFLP），其与基因型fliC-H8a和-H8b以及基因型fliC-H40a和-H40b相关。 fliC特异的PCR / RFLP分型方法适用于快速分型来自不同来源且其fliC-H8基因型高度保守的STEC O8，O22，O111，O174和O179菌株。 fliC基因分型方法优于血清分型，可用于流行病学调查和STEC克隆进化的研究。

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